Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, 37°C incubated mouse brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26122-1-AP targets GPM6B in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23507 Product name: Recombinant human GPM6B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-62 aa of BC047295 Sequence: MKPAMETAAEENTEQSQERKVNSRAEMEIGRYHWMYPGSKNHQYHPVPTLGDRASPLSSPGC 相同性解析による交差性が予測される生物種 |
| Full Name | glycoprotein M6B |
| Observed molecular weight | 27-36 kDa |
| GenBank accession number | BC047295 |
| Gene Symbol | GPM6B |
| Gene ID (NCBI) | 2824 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13491 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GPM6B (Glycoprotein M6B) is a highly conserved, multi-pass transmembrane glycoprotein encoded by the GPM6B gene on human chromosome Xp22.2. As a member of the proteolipid protein family, it is predominantly expressed in the central nervous system (in neurons and oligodendrocytes) and is involved in key processes such as myelination, regulation of serotonin transporter (SERT) trafficking, and actin cytoskeleton maintenance. Beyond neural functions, GPM6B also promotes bone formation by regulating osteoblast mineralization and influences smooth muscle cell differentiation via the TGF‑beta‑Smad2/3 pathway. Subcellularly, it localizes to the plasma membrane and lipid rafts. Genetic variations in GPM6B have been associated with several disorders, including Pelizaeus‑Merzbacher disease, major depressive disorder, and osteoarthritis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GPM6B antibody 26122-1-AP | Download protocol |
| IHC protocol for GPM6B antibody 26122-1-AP | Download protocol |
| IP protocol for GPM6B antibody 26122-1-AP | Download protocol |
| WB protocol for GPM6B antibody 26122-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |