Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87139-1-RR targets GPM6A in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag6915 Product name: Recombinant human GPM6A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-278 aa of BC022528 Sequence: MEENMEEGQTQKGCFECCIKCLGGIPYASLIATILLYAGVALFCGCGHEALSGTVNILQTYFEMARTAGDTLDVFTMIDIFKYVIYGIAAAFFVYGILLMVEGFFTTGAIKDLYGDFKITTCGRCVSAWFIMLTYLFMLAWLGVTAFTSLPVYMYFNLWTICRNTTLVEGANLCLDLRQFGIVTIGEEKKICTVSENFLRMCESTELNMTFHLFIVALAGAGAAVIAMVHYLMVLSANWAYVKDACRMQKYEDIKSKEEQELHDIHSTRSKERLNAYT 相同性解析による交差性が予測される生物種 |
| Full Name | glycoprotein M6A |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC022528 |
| Gene Symbol | GPM6A |
| Gene ID (NCBI) | 2823 |
| RRID | AB_3745365 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P51674 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glycoprotein M6A (GPM6A, or M6A) is known as a multi-pass transmembrane protein and an abundant cell surface protein on neurons in the central nervous system (CNS). M6A belongs to the myelin proteolipid protein family and is involved in neuronal differentiation, including differentiation and migration of neuronal stem cell. M6A is identified as a retinal membrane protein that is strongly expressed at embryonic stages. In mouse adult retina, M6A plays a role in neuronal plasticity and is involved in neurite and filopodia outgrowth, filopodia motility and probably synapse formation via regulation of MAPK and Src signaling pathways.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for GPM6A antibody 87139-1-RR | Download protocol |
| WB protocol for GPM6A antibody 87139-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |