Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human tonsil tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98942-1-RR targets GPI in IHC, FC (Intra) applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
unknow 相同性解析による交差性が予測される生物種 |
| Full Name | glucose phosphate isomerase |
| Gene Symbol | GPI |
| Gene ID (NCBI) | 2821 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
GPI (Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase (PGI), Neuroleukinis (NLK), phosphohexose isomerase (PHI) or sperm antigen 36 (SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GPI antibody 98942-1-RR | Download protocol |
| IHC protocol for GPI antibody 98942-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |