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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GPI Monoclonal antibody (67178-1-Ig)

    WB analysis of HeLa using 67178-1-Ig (same clone as 67178-1-PBS)

    WB result of GPI antibody (67178-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GPI Monoclonal antibody (67178-1-Ig)

    WB analysis using 67178-1-Ig (same clone as 67178-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67178-1-Ig (GPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GPI Monoclonal antibody (67178-1-Ig)

    WB analysis of HeLa using 67178-1-Ig (same clone as 67178-1-PBS)

    WB result of GPI antibody (67178-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPI transfected HeLa cells. This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GPI Monoclonal antibody (67178-1-Ig)

    IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using GPI Monoclonal antibody (67178-1-Ig)

    IHC staining of human lung cancer using 67178-1-Ig (same clone as 67178-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67178-1-Ig (GPI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using GPI Monoclonal antibody (67178-1-Ig)

    IF Staining of PC-3 using 67178-1-Ig (same clone as 67178-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using GPI antibody (67178-1-Ig, Clone: 2F11E4 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67178-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GPI Monoclonal antibody, PBS Only

GPI Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67178-1-PBS
Clone No.2F11E4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Neuroleukin, GNPI, Glucose-6-phosphate isomerase, EC:5.3.1.9, AMF

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67178-1-PBS targets GPI in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7419

Product name: Recombinant human GPI protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-272 aa of BC004982

Sequence: MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGG

相同性解析による交差性が予測される生物種
Full Name glucose phosphate isomerase
Calculated molecular weight 63 kDa
Observed molecular weight 55-64 kDa
GenBank accession numberBC004982
Gene Symbol GPI
Gene ID (NCBI) 2821
RRIDAB_2882474
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP06744
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GPI(Glucose-6-phosphate isomerase), which is also named as autocrine motility factor (AMF), phosphoglucose isomerase(PGI), Neuroleukinis(NLK), phosphohexose isomerase(PHI) or sperm antigen 36(SA-36), is a housekeeping cytosolic enzyme that plays a key role in both glycolysis and gluconeogenesis pathways. It is also a multifunctional protein that displays cytokine properties, eliciting mitogenic, motogenic, and differentiation activities, and has been implicated in tumor progression and metastasis (PMID:12783864, 19603112). This protein can exsit as a homodimer in the catalytically active form and a monomer in the secreted form (PMID:11371164). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 63-64kDa, and an apparent molecular mass of 55 and 64 kDa under non-reducing and reducing conditions, respectively(PMID: 19603112, 11004567).

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