Validation Data Gallery
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Tested Applications
| Positive WB detected in | rat liver tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
27943-1-AP targets GPD1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27703 Product name: Recombinant human GPD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 40-181 aa of BC032234 Sequence: VFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELM 相同性解析による交差性が予測される生物種 |
| Full Name | glycerol-3-phosphate dehydrogenase 1 (soluble) |
| Calculated molecular weight | 349 aa, 38 kDa |
| Observed molecular weight | 32-42 kDa |
| GenBank accession number | BC032234 |
| Gene Symbol | GPD1 |
| Gene ID (NCBI) | 2819 |
| RRID | AB_2881016 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P21695 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPD1 antibody 27943-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Saturation of the mitochondrial NADH shuttles drives aerobic glycolysis in proliferating cells | ||
Mol Cell Uncoupled glycerol-3-phosphate shuttle in kidney cancer reveals that cytosolic GPD is essential to support lipid synthesis
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