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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GPATCH4 Polyclonal antibody (24399-1-AP)

    WB analysis of HEK-293 using 24399-1-AP (same clone as 24399-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24399-1-AP (GPATCH4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24399-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using GPATCH4 Polyclonal antibody (24399-1-AP)

    WB analysis of mouse kidney using 24399-1-AP (same clone as 24399-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24399-1-AP (GPATCH4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24399-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using GPATCH4 Polyclonal antibody (24399-1-AP)

    WB analysis of rat kidney using 24399-1-AP (same clone as 24399-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 24399-1-AP (GPATCH4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24399-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GPATCH4 Polyclonal antibody (24399-1-AP)

    WB analysis of HEK-293 using 24399-1-AP (same clone as 24399-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24399-1-AP (GPATCH4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GPATCH4 Polyclonal antibody (24399-1-AP)

    IHC staining of human liver using 24399-1-AP (same clone as 24399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 24399-1-AP (GPATCH4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GPATCH4 Polyclonal antibody (24399-1-AP)

    IHC staining of human liver cancer using 24399-1-AP (same clone as 24399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 24399-1-AP (GPATCH4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24399-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GPATCH4 Polyclonal antibody (24399-1-AP)

    IHC staining of human liver using 24399-1-AP (same clone as 24399-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 24399-1-AP (GPATCH4 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24399-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using GPATCH4 Polyclonal antibody (24399-1-AP)

    IF Staining of HEK-293 using 24399-1-AP (same clone as 24399-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 24399-1-AP (GPATCH4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24399-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24399-1-PBS targets GPATCH4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPATCH4 fusion protein Ag20095 相同性解析による交差性が予測される生物種
Full Name G patch domain containing 4
Calculated molecular weight 446 aa, 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC040147
Gene Symbol GPATCH4
Gene ID (NCBI) 54865
RRIDAB_2879523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T3I0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GPATCH4 (G patch domain-containing protein 4) is a 446 amino acid protein containing one G-patch domain. Existing as three alternatively spliced isoforms. This antibody is a rabbit polyclonal antibody raised against the 189 residues of N-terminal of human GPATCH4 protein