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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPAM Recombinant antibody (84054-5-RR)

    WB analysis using 84054-5-RR (same clone as 84054-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84054-5-RR (GPAM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84054-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GPAM Recombinant antibody (84054-5-RR)

    WB analysis of HepG2 using 84054-5-RR (same clone as 84054-5-PBS)

    WB result of GPAM antibody (84054-5-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPAM transfected HepG2 cells. This data was developed using the same antibody clone with 84054-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84054-5-PBS

    Affinity and Kinetic Characterization of 84054-5-PBS

    Biolayer interferometry (BLl) kinetic assay of 84054-5-PBS against Human GPAM was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 84054-5-RR

    Affinity and Kinetic Characterization of 84054-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84054-5-RR against Human GPAM were performed. The affinity constant is below 1 pM.

GPAM Recombinant monoclonal antibody, PBS Only

GPAM Uni-rAb® Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 84054-5-PBS
Clone No.241144H6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

241144H6, EC:2.3.1.15, Glycerol-3-phosphate acyltransferase 1, mitochondrial, GPAT 1, GPAT1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84054-5-PBS targets GPAM in WB, Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3120

Product name: Recombinant human GPAM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 479-828 aa of BC030783

Sequence: SCAIMSTHIVACLLLYRHRQGIDLSTLVEDFFVMKEEVLARDFDLGFSGNSEDVVMHAIQLLGNCVTITHTSRNDEFFITPSTTVPSVFELNFYSNGVLHVFIMEAIIACSLYAVLNKRGLGGPTSTPPNLISQEQLVRKAASLCYLLSNEGTISLPCQTFYQVCHETVGKFIQYGILTVAEHDDQEDISPSLAEQQWDKKLPEPLSWRSDEEDEDSDFGEEQRDCYLKVSQSKEHQQFITFLQRLLGPLLEAYSSAAIFVHNFSGPVPEPEYLQKLHKYLITRTERNVAVYAESATYCLVKNAVKMFKDIGVFKETKQKRVSVLELSSTFLPQCNRQKLLEYILSFVVL

相同性解析による交差性が予測される生物種
Full Name glycerol-3-phosphate acyltransferase, mitochondrial
Calculated molecular weight 828 aa, 94 kDa
Observed molecular weight 90-92 kDa
GenBank accession numberBC030783
Gene Symbol GPAM
Gene ID (NCBI) 57678
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9HCL2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GPAM (Glycerol-3-Phosphate Acyltransferase, Mitochondrial) is a Protein Coding gene. This gene encodes a mitochondrial enzyme which prefers saturated fatty acids as its substrate for the synthesis of glycerolipids. This metabolic pathway's first step is catalyzed by the encoded enzyme. Two forms for this enzyme exist, one in the mitochondria and one in the endoplasmic reticulum. Two alternatively spliced transcript variants have been described for this gene.Diseases associated with GPAM include Rhizomelic Chondrodysplasia Punctata, Type 2 and Congenital Generalized Lipodystrophy. GPAM can be detected as 90-92 kDa.