Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, HT-29 cells, mouse colon tissue, mouse small intestine tissue, rat colon tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25941-1-AP targets GPA33 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12655 Product name: Recombinant human GPA33 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-319 aa of BC074830 Sequence: TPQDVLRASQGKSVTLPCTYHTSTSSREGLIQWDKLLLTHTERVVIWPFSNKNYIHGELYKNRVSISNNAEQSDASITIDQLTMADNGTYECSVSLMSDLEGNTKSRVRLLVLVPPSKPECGIEGETIIGNNIQLTCQSKEGSPTPQYSWKRYNILNQEQPLAQPASGQPVSLKNISTDTSGYYICTSSNEEGTQFCNITVAVRSPSMNVALYVGIAVGVVAALIIIGIIIYCCCCRGKDDNTEDKEDARPNREAYEEPPEQLRELSREREEEDDYRQEEQRSTGRESPDHLDQ 相同性解析による交差性が予測される生物種 |
| Full Name | glycoprotein A33 (transmembrane) |
| Calculated molecular weight | 319 aa, 36 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC074830 |
| Gene Symbol | GPA33 |
| Gene ID (NCBI) | 10223 |
| RRID | AB_3669509 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q99795 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Glycoprotein A33 (GPA33) is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 9862345; 9012807). It contains an extracellular region of 213 amino acids, a transmembrane domain, and a highly polar intracellular tail containing four consecutive cysteine residues (PMID: 9012807). GPA33 is specifically expressed on the basolateral surfaces of intestinal epithelial cells of all lineages and is found in 95% of primary and metastatic colorectal cancers (PMID: 10960348; 10950144; 21541536). GPA33 may have a role in cell adhesion and has been implicated in immune dysregulation (PMID: 9012807; 33728639).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GPA33 antibody 25941-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |