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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GPA33 Polyclonal antibody (25941-1-AP)

    WB analysis using 25941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25941-1-AP (GPA33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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GPA33 Polyclonal antibody

GPA33 Polyclonal Antibody for WB, ELISA
Cat No. 25941-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

A33, Cell surface A33 antigen, Glycoprotein A33, GPA33

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCOLO 320 cells, HT-29 cells, mouse colon tissue, mouse small intestine tissue, rat colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25941-1-AP targets GPA33 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPA33 fusion protein Ag12655 相同性解析による交差性が予測される生物種
Full Name glycoprotein A33 (transmembrane)
Calculated molecular weight 319 aa, 36 kDa
Observed molecular weight50 kDa
GenBank accession numberBC074830
Gene Symbol GPA33
Gene ID (NCBI) 10223
RRIDAB_3669509
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99795
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glycoprotein A33 (GPA33) is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily (PMID: 9862345; 9012807). It contains an extracellular region of 213 amino acids, a transmembrane domain, and a highly polar intracellular tail containing four consecutive cysteine residues (PMID: 9012807). GPA33 is specifically expressed on the basolateral surfaces of intestinal epithelial cells of all lineages and is found in 95% of primary and metastatic colorectal cancers (PMID: 10960348; 10950144; 21541536). GPA33 may have a role in cell adhesion and has been implicated in immune dysregulation (PMID: 9012807; 33728639).

Protocols

Product Specific Protocols
WB protocol for GPA33 antibody 25941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols