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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GP2 Polyclonal antibody (13493-1-AP)

    WB analysis using 13493-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13493-1-AP (GP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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GP2 Polyclonal antibody

GP2 Polyclonal Antibody for WB, ELISA
Cat No. 13493-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ZAP75, Pancreatic zymogen granule membrane protein GP-2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat pancreas tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13493-1-AP targets GP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GP2 fusion protein Ag4317 相同性解析による交差性が予測される生物種
Full Name glycoprotein 2 (zymogen granule membrane)
Calculated molecular weight 530 aa, 58 kDa
Observed molecular weight75-85 kDa
GenBank accession numberBC032693
Gene Symbol GP2
Gene ID (NCBI) 2813
RRIDAB_3669164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55259
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pancreatic secretory granule membrane major glycoprotein (GP2) is the most abundant membrane component of zymogen granules secreted by pancreatic acinar cells (PMID: 8190715). GP2 associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. It is released from the membrane of mature zymogen granules by an enzymatic mechanism (PMID: 2011597). GP2 plays a role in defense against adhesive and invasive commensal bacteria during intestinal inflammation (PMID: 33594081). GP2 is preferentially expressed in acinar cell carcinomas of the pancreas but the glycoprotein can rarely also be expressed in a variety of other tumor entities (PMID: 36137400). The apparent molecular weight of GP2 is 70-100 kDa, which is larger than the calculated molecular weight, due to glycosylation (PMID: 19627615; 30186250).

Protocols

Product Specific Protocols
WB protocol for GP2 antibody 13493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols