Validation Data Gallery
Tested Applications
| Positive WB detected in | Human peripheral blood platelets, pig peripheral blood leukocyte, human blood |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 21 publications below |
| IHC | See 14 publications below |
| IF | See 4 publications below |
| ELISA | See 1 publications below |
Product Information
12860-1-AP targets CD42b in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3893 Product name: Recombinant human GP1BA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-350 aa of BC027955 Sequence: ICEVSKVASHLEVNCDKRNLTALPPDLPKDTTILHLSENLLYTFSLATLMPYTRLTQLNLDRCELTKLQVDGTLPVLGTLDLSHNQLQSLPLLGQTLPALTVLDVSFNRLTSLPLGALRGLGELQELYLKGNELKTLPPGLLTPTPKLEKLSLANNNLTELPAGLLNGLENLDTLLLQENSLYTIPKGFFGSHLLPFAFLHGNPWLCNCEILYFRRWLQDNAENVYVWKQGVDVKAMTSNVASVQCDNSDKFPVYKYPGKGCPTLGDEGDTDLYDYYPEEDTEGDKVRATRTVVKFPTKAHTTPWGLFYSWSTASLDSQMPSSLHPTQESTK 相同性解析による交差性が予測される生物種 |
| Full Name | glycoprotein Ib (platelet), alpha polypeptide |
| Calculated molecular weight | 626 aa, 69 kDa |
| Observed molecular weight | 145 kDa |
| GenBank accession number | BC027955 |
| Gene Symbol | CD42b |
| Gene ID (NCBI) | 2811 |
| ENSEMBL Gene ID | ENSG00000185245 |
| RRID | AB_10644481 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07359 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GP1BA, also named as CD42b , is a 145-kDa surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. It has a heterodimer form which composed of GP1BA and GP1BB.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD42b antibody 12860-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Sequential simulation of regeneration-specific microenvironments using scaffolds loaded with nanoplatelet vesicles enhances bone regeneration | ||
ACS Nano Cryo-Shocked Platelet Coupled with ROS-Responsive Nanomedicine for Targeted Treatment of Thromboembolic Disease | ||
Trends Biotechnol A regenerative and programmable iPSC-derived platelet platform for universal drug delivery and immunotherapy. | ||
Sci Transl Med Dietary ketone body-escalated histone acetylation in megakaryocytes alleviates chemotherapy-induced thrombocytopenia | ||
J Nanobiotechnology Platelet-rich plasma-derived extracellular vesicles delivered niraparib for ultrasound imaging and atherosclerosis treatment. |