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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GORASP2 Recombinant antibody (85077-5-RR)

    WB analysis using 85077-5-RR (same clone as 85077-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85077-5-RR (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GORASP2 Recombinant antibody (85077-5-RR)

    IHC staining of human colon cancer using 85077-5-RR (same clone as 85077-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85077-5-RR (GORASP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GORASP2 Recombinant antibody (85077-5-RR)

    IHC staining of human colon cancer using 85077-5-RR (same clone as 85077-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85077-5-RR (GORASP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GORASP2 Recombinant antibody (85077-5-RR)

    IF Staining of HepG2 using 85077-5-RR (same clone as 85077-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GORASP2 Recombinant antibody (85077-5-RR)

    IF Staining of HepG2 using 85077-5-RR (same clone as 85077-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85077-5-RR

    Affinity and Kinetic Characterization of 85077-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85077-5-RR against Human GORASP2 were performed. The affinity constant is 5.33 nM.

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GORASP2 Recombinant antibody, PBS Only

GORASP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85077-5-PBS
Clone No.242700C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2, Golgi phosphoprotein 6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85077-5-PBS targets GORASP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GORASP2 fusion protein Ag0950 相同性解析による交差性が予測される生物種
Full Name golgi reassembly stacking protein 2, 55kDa
Calculated molecular weight 55 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC007770
Gene Symbol GORASP2
Gene ID (NCBI) 26003
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H8Y8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.