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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLPH3L Polyclonal antibody (23153-1-AP)

    WB analysis using 23153-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23153-1-AP (GOLPH3L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GOLPH3L Polyclonal antibody (23153-1-AP)

    IHC staining of human cervical cancer using 23153-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23153-1-AP (GOLPH3L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GOLPH3L Polyclonal antibody (23153-1-AP)

    IHC staining of human cervical cancer using 23153-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 23153-1-AP (GOLPH3L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GOLPH3L Polyclonal antibody

GOLPH3L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23153-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, SK-BR-3 cells, T-47D cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23153-1-AP targets GOLPH3L in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOLPH3L fusion protein Ag19537 相同性解析による交差性が予測される生物種
Full Name golgi phosphoprotein 3-like
Calculated molecular weight 285 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC013870
Gene Symbol GOLPH3L
Gene ID (NCBI) 55204
RRIDAB_3669408
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4A5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Golgi phosphoprotein 3 (GOLPH3) is a highly conserved membrane protein that plays an important role in maintaining the structure of the Golgi apparatus and anterograde transport. GOLPH3L(golgi phosphoprotein 3-like) is a GOLPH3 paralogue found in all vertebrate genomes. Compared with the oncogene GOLPH3, little is known about the role and function of GOLPH3L. It has been reported that GOLPH3L is highly expressed in epithelial ovarian cancer tissues, and cervical cancer tissue and is up-regulated in human rhabdomyosarcoma (PMID: 26284141; 28261346; 21822541).

Protocols

Product Specific Protocols
WB protocol for GOLPH3L antibody 23153-1-APDownload protocol
IHC protocol for GOLPH3L antibody 23153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols