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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLIM4 Polyclonal antibody (31083-1-AP)

    WB analysis using 31083-1-AP (same clone as 31083-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31083-1-AP (GOLIM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GOLIM4 Polyclonal antibody (31083-1-AP)

    WB analysis of HEK-293 using 31083-1-AP (same clone as 31083-1-PBS)

    WB result of GOLIM4 antibody (31083-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLIM4 transfected HEK-293 cells. This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GOLIM4 Polyclonal antibody (31083-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 31083-1-AP (same clone as 31083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31083-1-AP (GOLIM4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GOLIM4 Polyclonal antibody (31083-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 31083-1-AP (same clone as 31083-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 31083-1-AP (GOLIM4 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLIM4 Polyclonal antibody (31083-1-AP)

    IF Staining of HeLa using 31083-1-AP (same clone as 31083-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLIM4 antibody (31083-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLIM4 Polyclonal antibody (31083-1-AP)

    IF Staining of HeLa using 31083-1-AP (same clone as 31083-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLIM4 antibody (31083-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 31083-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GOLIM4 Polyclonal antibody, PBS Only

GOLIM4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31083-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

GIMPC, GOLPH4, GPP130, P 138, P138

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31083-1-PBS targets GOLIM4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34962

Product name: Recombinant human GOLIM4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 350-500 aa of NM_014498

Sequence: QVGQAEHLEEEHDPSPEEQDREWKEQHEQREAANLLEGHARAEVYPSAKPMIKFQSPYEEQLEQQRLAVQQVEEAQQLREHQEALHQQRLQGHLLRQQEQQQQQVAREMALQRQAELEEGRPQHQEQLRQQAHYDAMDNDIVQGAEDQGIQ

相同性解析による交差性が予測される生物種
Full Name golgi integral membrane protein 4
Calculated molecular weight82 kDa
Observed molecular weight130 kDa
GenBank accession numberNM_014498
Gene Symbol GOLIM4
Gene ID (NCBI) 27333
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDO00461
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Golgi integral membrane protein 4 (GOLIM4) is a 130-kDa membrane protein that is integrated into the cis/medial Golgi apparatus, which is a kind II Golgi protein. GOLIM4 is a Golgi protein that circulates between Golgi apparatus and endosomes, and its shuttle is pH sensitive(PMID:30068697).

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