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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLGA7 Polyclonal antibody (14723-1-AP)

    WB analysis using 14723-1-AP (same clone as 14723-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14723-1-AP (GOLGA7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14723-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GOLGA7 Polyclonal antibody (14723-1-AP)

    WB analysis of HEK-293 using 14723-1-AP (same clone as 14723-1-PBS)

    WB result of GOLGA7 antibody (14723-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA7 transfected HEK-293 cells. This data was developed using the same antibody clone with 14723-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using GOLGA7 Polyclonal antibody (14723-1-AP)

    IHC staining of mouse stomach using 14723-1-AP (same clone as 14723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14723-1-AP (GOLGA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14723-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using GOLGA7 Polyclonal antibody (14723-1-AP)

    IHC staining of mouse stomach using 14723-1-AP (same clone as 14723-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 14723-1-AP (GOLGA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14723-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GOLGA7 Polyclonal antibody, PBS Only

GOLGA7 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14723-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14723-1-PBS targets GOLGA7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6460

Product name: Recombinant human GOLGA7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-137 aa of BC012032

Sequence: MRPQQAPVSGKVFIQRDYSSGTRCQFQTKFPAELENRIDRQQFEETVRTLNNLYAEAEKLGGQSYLEGCLACLTAYTIFLCMETHYEKVLKKVSKYIQEQNEKIYAPQGLLLTDPIERGLRVIEITIYEDRGMSSGR

相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 7
Calculated molecular weight 16 kDa
Observed molecular weight15-20 kDa
GenBank accession numberBC012032
Gene Symbol GOLGA7
Gene ID (NCBI) 51125
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z5G4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GOLGA7 (Golgin subfamily A member 7) is an acylated Golgi protein that interacts with GCP170 protein (PMID: 14522980). The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS. The ZDHHC5-GOLGA7 protein acyltransferase complex promotes nonapoptotic cell death (PMID: 31631010).

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