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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLGA5 Polyclonal antibody (31164-1-AP)

    WB analysis using 31164-1-AP (same clone as 31164-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31164-1-AP (GOLGA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31164-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GOLGA5 Polyclonal antibody (31164-1-AP)

    IF Staining of HepG2 using 31164-1-AP (same clone as 31164-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GOLGA5 antibody (31164-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31164-1-PBS in a different storage buffer formulation.

GOLGA5 Polyclonal antibody, PBS Only

GOLGA5 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31164-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31164-1-PBS targets GOLGA5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag35031

Product name: Recombinant human GOLGA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 251-410 aa of BC023021

Sequence: RSKETQEELNKARARVEKWNADHSKSDRMTRGLRAQVDDLTEAVAAKDSQLAVLKVRLQEADQLLSTRTEALEALQSEKSRIMQDQSEGNSLQNQALQTLQERLHEADATLKREQESYKQMQSEFAARLNKVEMERQNLAEAITLAERKYSDEKKRVDEL

相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 5
Calculated molecular weight 83 kDa
Observed molecular weight 83-95 kDa
GenBank accession numberBC023021
Gene Symbol GOLGA5
Gene ID (NCBI) 9950
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8TBA6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GOLGA5 (Golgin subfamily A member 5) maintains Golgi structure, and stimulates the formation of Golgi stacks and ribbons. Also, it is involved in intra-Golgi retrograde transport (PMID: 12538640; 15718469).

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