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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLGA4 Polyclonal antibody (55207-1-AP)

    WB analysis using 55207-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55207-1-AP (GOLGA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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GOLGA4 Polyclonal antibody

GOLGA4 Polyclonal Antibody for WB, ELISA
Cat No. 55207-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

MU RMS 40.18, Golgin subfamily A member 4, Golgin 245, GOLG, GCP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inT-47D cells, U-118 MG cells, Neuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55207-1-AP targets GOLGA4 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 4
Calculated molecular weight 161 kDa
Observed molecular weight260-270 kDa
GenBank accession numberNM_002078
Gene Symbol GOLGA4
Gene ID (NCBI) 2803
RRIDAB_3670242
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13439
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GOLGA4, also known as Golgi matrix protein, is a member of the Golgin family and is mainly localized in the Golgi apparatus. It is involved in vesicle transport at the Golgi level and may play a role in the transport of GPI-anchored proteins from the Golgi network. GOLGA4 is expressed in a variety of tissues, especially in the testis, but its specific function in spermatogenesis and male fertility is not fully understood. Studies have shown that GOLGA4 knockout male mice are still fertile, and the testis and sperm morphology are normal, indicating that GOLGA4 is not essential for mouse spermatogenesis and male fertility (PMID: 32736686).

Protocols

Product Specific Protocols
WB protocol for GOLGA4 antibody 55207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols