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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLGA3 Recombinant antibody (84955-4-RR)

    WB analysis using 84955-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84955-4-RR (GOLGA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLGA3 Recombinant antibody (84955-4-RR)

    IF Staining of HeLa using 84955-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA3 antibody (84955-4-RR, Clone: 242603C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84955-4-RR

    Affinity and Kinetic Characterization of 84955-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84955-4-RR against Human GOLGA3 were performed. The affinity constant is 0.475 nM.

GOLGA3 Recombinant monoclonal antibody

GOLGA3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84955-4-RR
Clone No.242603C7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

GCP170, Golgi complex-associated protein of 170 kDa, Golgin 160, Golgin-160, MEA 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, L02 cells, PC-3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84955-4-RR targets GOLGA3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag15531

Product name: Recombinant human GOLGA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC142658

Sequence: MDGASAEQDGLQEDRSHSGPSSLPEAPLKPPGPLVPPDQQDKVQCAEVNRASTEGESPDGPGQGGLCQNGPTPPFPDPPSSLDPTTSPVGPDASPGVAGFHDNLRKSQGTSAEGSVRKEALQSLRLSLPMQETQLCSTDSPLPLEKEEQVRLQARKWLEEQLKQYRVKRQQERSSQPATKTRLFSTLDPELMLNPENLPRASTLAMTKEYSFLRTSVPRGPKVGSLGLPAHPREKKTSKSSKIRSLADYRTEDSNAGNSGGNVPAPDSTKGSLKQNRSSAASVVSEISLSPDTDDRLENTSLAGDSVSEVDGNDSDSSSYSSASTRGTYGILSKTVGTQDTPYMVNGQEI

相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 3
Calculated molecular weight 1498 aa, 167 kDa
Observed molecular weight 167 kDa
GenBank accession numberBC142658
Gene Symbol GOLGA3
Gene ID (NCBI) 2802
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ08378
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Golgin subfamily A member 3 (Golga3), a member of the golgin subfamily A, is highly expressed in mouse testis. The GOLGA3 protein, which contains eight phosphorylation sites, is involved in protein transport, cell apoptosis, Golgi localization, and spermatogenesis.

Protocols

Product Specific Protocols
IF protocol for GOLGA3 antibody 84955-4-RRDownload protocol
WB protocol for GOLGA3 antibody 84955-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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