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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    WB analysis using 11308-1-AP (same clone as 11308-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    WB analysis of HeLa using 11308-1-AP (same clone as 11308-1-PBS)

    WB result of GOLGA2/GM130 antibody (11308-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    WB analysis of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)

    WB result of GOLGA2/GM130 antibody (11308-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    WB analysis using 11308-1-AP (same clone as 11308-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    WB analysis of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)

    HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human spleen tissue using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    WB analysis of human spleen using 11308-1-AP (same clone as 11308-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    IHC staining of human testis using 11308-1-AP (same clone as 11308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    IHC staining of human testis using 11308-1-AP (same clone as 11308-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    IF Staining of HepG2 using 11308-1-AP (same clone as 11308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    IF Staining of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    IF Staining of HepG2 using 11308-1-AP (same clone as 11308-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    IF Staining of HeLa using 11308-1-AP (same clone as 11308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    IF Staining of HeLa using 11308-1-AP (same clone as 11308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    IF Staining of MDCK using 11308-1-AP (same clone as 11308-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using GOLGA2/GM130 Polyclonal antibody (11308-1-AP)

    FC experiment of HepG2 using 11308-1-AP (same clone as 11308-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.2 ug GOLGA2/GM130 Polyclonal antibody (11308-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

GOLGA2/GM130 Polyclonal antibody, PBS Only

GOLGA2/GM130 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 11308-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

GM130, GOLGA2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, GM130;GOLGA2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11308-1-PBS targets GOLGA2/GM130 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity human, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GOLGA2/GM130 fusion protein Ag1848 相同性解析による交差性が予測される生物種
Full Name golgi autoantigen, golgin subfamily a, 2
Calculated molecular weight 111 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC014188
Gene Symbol GM130
Gene ID (NCBI) 2801
RRIDAB_2115327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08379
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)