Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 11308-1-AP (same clone as 11308-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 11308-1-AP (same clone as 11308-1-PBS)
WB result of GOLGA2/GM130 antibody (11308-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)
WB result of GOLGA2/GM130 antibody (11308-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
WB analysis using 11308-1-AP (same clone as 11308-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)
HEK-293 cells and HEK-293-derived exosomes (HEK-293-exo) were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
WB analysis of human spleen using 11308-1-AP (same clone as 11308-1-PBS)
human spleen tissue were subjected to SDS PAGE followed by western blot with 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 11308-1-AP (same clone as 11308-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 11308-1-AP (same clone as 11308-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 11308-1-AP (GOLGA2,GM130 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 11308-1-AP (same clone as 11308-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 11308-1-AP (same clone as 11308-1-PBS)
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 11308-1-AP (same clone as 11308-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 11308-1-AP (same clone as 11308-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (11308-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
IF Staining of MDCK using 11308-1-AP (same clone as 11308-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed MDCK cells using 11308-1-AP (GOLGA2/GM130 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 11308-1-AP (same clone as 11308-1-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.2 ug GOLGA2/GM130 Polyclonal antibody (11308-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.
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1x10^6 HepG2 cells were intracellularly stained with 0.2 ug GOLGA2/GM130 Polyclonal antibody (11308-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11308-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

GOLGA2/GM130 Polyclonal antibody, PBS Only

GOLGA2/GM130 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 11308-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, canine

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

GM130, GOLGA2, 130 kDa cis-Golgi matrix protein, GM130 autoantigen, GM130;GOLGA2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11308-1-PBS targets GOLGA2/GM130 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, canine samples.

Tested Reactivity	human, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI 相同性解析による交差性が予測される生物種
Full Name	golgi autoantigen, golgin subfamily a, 2
Calculated molecular weight	111 kDa
Observed molecular weight	130 kDa
GenBank accession number	BC014188
Gene Symbol	GM130
Gene ID (NCBI)	2801
RRID	AB_2115327
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q08379
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11308-1-AP (same clone as 11308-1-PBS)

WB analysis of HeLa using 11308-1-AP (same clone as 11308-1-PBS)

WB analysis of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)

WB analysis using 11308-1-AP (same clone as 11308-1-PBS)

WB analysis of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)

WB analysis of human spleen using 11308-1-AP (same clone as 11308-1-PBS)

IHC staining of human testis using 11308-1-AP (same clone as 11308-1-PBS)

IHC staining of human testis using 11308-1-AP (same clone as 11308-1-PBS)

IF Staining of HepG2 using 11308-1-AP (same clone as 11308-1-PBS)

IF Staining of HEK-293 using 11308-1-AP (same clone as 11308-1-PBS)