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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using GNS Polyclonal antibody (13044-1-AP)

    WB analysis of PC-3 using 13044-1-AP (same clone as 13044-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 13044-1-AP (GNS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GNS Polyclonal antibody (13044-1-AP)

    WB analysis of HeLa using 13044-1-AP (same clone as 13044-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13044-1-AP (GNS antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GNS Polyclonal antibody (13044-1-AP)

    IHC staining of human prostate cancer using 13044-1-AP (same clone as 13044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13044-1-AP (GNS antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using GNS Polyclonal antibody (13044-1-AP)

    IHC staining of human prostate cancer using 13044-1-AP (same clone as 13044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13044-1-AP (GNS antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13044-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using GNS Polyclonal antibody (13044-1-AP)

    IF Staining of PC-3 using 13044-1-AP (same clone as 13044-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 13044-1-AP (GNS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13044-1-PBS in a different storage buffer formulation.

GNS Polyclonal antibody, PBS Only

GNS Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13044-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13044-1-PBS targets GNS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3712

Product name: Recombinant human GNS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 200-552 aa of BC012482

Sequence: SVDYLTDVLANVSLDFLDYKSNFEPFFMMIATPAPHSPWTAAPQYQKAFQNVFAPRNKNFNIHGTNKHWLIRQAKTPMTNSSIQFLDNAFRKRWQTLLSVDDLVEKLVKRLEFTGELNNTYIFYTSDNGYHTGQFSLPIDKRQLYEFDIKVPLLVRGPGIKPNQTSKMLVANIDLGPTILDIAGYDLNKTQMDGMSLLPILRGASNLTWRSDVLVEYQGEGRNVTDPTCPSLSPGVSQCFPDCVCEDAYNNTYACVRTMSALWNLQYCEFDDQEVFVEVYNLTADPDQITNIAKTIDPELLGKMNYRLMMLQSCSGPTCRTPGVFDPGYRFDPRLMFSNRGSVRTRRFSKHLL

相同性解析による交差性が予測される生物種
Full Name glucosamine (N-acetyl)-6-sulfatase
Calculated molecular weight 552 aa, 62 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC012482
Gene Symbol GNS
Gene ID (NCBI) 2799
RRIDAB_2115308
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP15586
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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