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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNPTG Polyclonal antibody (14074-1-AP)

    WB analysis using 14074-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14074-1-AP (GNPTG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GNPTG Polyclonal antibody (14074-1-AP)

    WB analysis using 14074-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14074-1-AP (GNPTG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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GNPTG Polyclonal antibody

GNPTG Polyclonal Antibody for WB, ELISA
Cat No. 14074-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

N-acetylglucosamine-1-phosphotransferase subunit gamma, LP2537, GNPTAG, GlcNAc-1-phosphotransferase subunit gamma, CAB56184

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRAW 264.7 cells, mouse testis tissue, rat testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14074-1-AP targets GNPTG in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNPTG fusion protein Ag5153 相同性解析による交差性が予測される生物種
Full Name N-acetylglucosamine-1-phosphate transferase, gamma subunit
Calculated molecular weight 34 kDa
Observed molecular weight34 kDa, 68~70 kDa
GenBank accession numberBC014592
Gene Symbol GNPTG
Gene ID (NCBI) 84572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJJ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GlcNAc-phosphotransferase is a multisubunit enzyme with an α2β2γ2 arrangement that requires a detergent for solubilization. Recent cloning of cDNAs and genes encoding these subunits revealed that the α- and β-subunits are encoded by a single gene as a precursor, whereas the γ-subunit is encoded by a second gene. Sugar composition and/ or additional modifications which alter the charge of the GNPTAG oligosaccharide chains are variable in a cell type-depending manner.

Protocols

Product Specific Protocols
WB protocol for GNPTG antibody 14074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols