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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GNPAT Polyclonal antibody (14931-1-AP)

    WB analysis of HepG2 using 14931-1-AP

    WB result of GNPAT antibody (14931-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNPAT transfected HepG2 cells.

  • Western Blot (WB) analysis of HepG2 cells using GNPAT Polyclonal antibody (14931-1-AP)

    WB analysis of HepG2 using 14931-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using GNPAT Polyclonal antibody (14931-1-AP)

    WB analysis of human placenta using 14931-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using GNPAT Polyclonal antibody (14931-1-AP)

    WB analysis of Transfected HEK-293 using 14931-1-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GNPAT Polyclonal antibody (14931-1-AP)

    WB analysis of mouse brain using 14931-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GNPAT Polyclonal antibody (14931-1-AP)

    WB analysis of HeLa using 14931-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GNPAT Polyclonal antibody (14931-1-AP)

    WB analysis of PC-3 using 14931-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using GNPAT Polyclonal antibody (14931-1-AP)

    WB analysis of COLO 320 using 14931-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14931-1-AP (GNPAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of COLO 320 cells using GNPAT Polyclonal antibody (14931-1-AP)

    IP experiment of COLO 320 using 14931-1-AP

    IP result of anti-GNPAT (IP:14931-1-AP, 4ug; Detection:14931-1-AP 1:500) with COLO 320 cells lysate 2400ug.

  • Featured Product
  • KD/KO Validated

GNPAT Polyclonal antibody

GNPAT Polyclonal Antibody for WB, IP, ELISA
Cat No. 14931-1-AP
Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

DAP AT, DAPAT, DHAP AT, DHAPAT, GNPAT

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, human placenta tissue, Transfected HEK-293 cells, COLO 320 cells, HeLa cells, PC-3 cells, mouse brain tissue
Positive IP detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14931-1-AP targets GNPAT in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6735

Product name: Recombinant human GNPAT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 331-680 aa of BC000450

Sequence: PVSLRSLAAGRMSRSSYNLVPRYIPQKQSEDMHAFVTEVAYKMELLQIENMVLSPWTLIVAVLLQNRPSMDFDALVEKTLWLKGLTQAFGGFLIWPDNKPAEEVVPASILLHSNIASLVKDQVILKVDSGDSEVVDGLMLQHITLLMCSAYRNQLLNIFVRPSLVAVALQMTPGFRKEDVYSCFRFLRDVFADEFIFLPGNTLKDFEEGCYLLCKSEAIQVTTKDILVTEKGNTVLEFLVGLFKPFVESYQIICKHLLSEEEDHFSEEQYLAAVRKFTSQLLDQGTSQCYDVLSSDVQKNALAACVRLGVVEKKKINNNCIFNVNEPATTKLEEMLGCKTPIGKPATAKL

相同性解析による交差性が予測される生物種
Full Name glyceronephosphate O-acyltransferase
Calculated molecular weight 77 kDa
Observed molecular weight 65-69 kDa
GenBank accession numberBC000450
Gene Symbol GNPAT
Gene ID (NCBI) 8443
RRIDAB_2110546
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15228
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS. (PMID:21990100) This antibody recognize the 69 kDa GNPAT protein.

Protocols

Product Specific Protocols
IP protocol for GNPAT antibody 14931-1-APDownload protocol
WB protocol for GNPAT antibody 14931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Host Microbe

Infection-Induced Peroxisome Biogenesis Is a Metabolic Strategy for Herpesvirus Replication.

Authors - Pierre M Jean Beltran
  • KD Validated
View Article
mouseWB

Nat Cell Biol

Functional multi-organelle units control inflammatory lipid metabolism of macrophages

Authors - Julia A Zimmermann
View Article
humanWB

Nat Commun

Ether phospholipids are required for mitochondrial reactive oxygen species homeostasis

Authors - Ziheng Chen
  • KO Validated
View Article

Mol Cell

Uridine cytidine kinase 1 promotes phosphatidylcholine biosynthesis, facilitating SARS-CoV-2 infection.

Authors - Guangda Shi
View Article
mouseWB

J Clin Invest

Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.

Authors - Hongsuk Park
View Article
human,mouseWB

Sci Adv

Bacterial pathogens hijack host cell peroxisomes for replication vacuole expansion and integrity

Authors - Mohammad J Hossain
View Article
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