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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNMT Monoclonal antibody (67294-1-Ig)

    WB analysis using 67294-1-Ig (same clone as 67294-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using GNMT Monoclonal antibody (67294-1-Ig)

    WB analysis of pig liver using 67294-1-Ig (same clone as 67294-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using GNMT Monoclonal antibody (67294-1-Ig)

    WB analysis of rat liver using 67294-1-Ig (same clone as 67294-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GNMT Monoclonal antibody (67294-1-Ig)

    WB analysis of mouse liver using 67294-1-Ig (same clone as 67294-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 67294-1-Ig (GNMT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GNMT Monoclonal antibody (67294-1-Ig)

    IF Staining of A431 using 67294-1-Ig (same clone as 67294-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GNMT antibody (67294-1-Ig, Clone: 1B5E3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GNMT Monoclonal antibody (67294-1-Ig)

    IF Staining of A431 using 67294-1-Ig (same clone as 67294-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GNMT antibody (67294-1-Ig, Clone: 1B5E3 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67294-1-PBS in a different storage buffer formulation.

GNMT Monoclonal antibody, PBS Only

GNMT Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67294-1-PBS
Clone No.1B5E3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EC:2.1.1.20, glycine N methyltransferase, Glycine N-methyltransferase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67294-1-PBS targets GNMT in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4598

Product name: Recombinant human GNMT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-295 aa of BC032627

Sequence: MVDSVYRTRSLGVAAEGLPDQYADGEAARVWQLYIGDTRSRTAEYKAWLLGLLRQHGCQRVLDVACGTGVDSIMLVEEGFSVTSVDASDKMLKYALKERWNRRHEPAFDKWVIEEANWMTLDKDVPQSAEGGFDAVICLGNSFAHLPDCKGDQSEHRLALKNIASMVRAGGLLVIDHRNYDHILSTGCAPPGKNIYYKSDLTKDVTTSVLIVNNKAHMVTLDYTVQVPGAGQDGSPGLSKFRLSYYPHCLASFTELLQAAFGGKCQHSVLGDFKPYKPGQTYIPCYFIHVLKRTD

相同性解析による交差性が予測される生物種
Full Name glycine N-methyltransferase
Calculated molecular weight 295 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC032627
Gene Symbol GNMT
Gene ID (NCBI) 27232
RRIDAB_2882559
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14749
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Glycine N-methyltransferase (GNMT, EC 2.1.1.20) was found originally as an enzyme regulating the ratio of SAM to S-adenosyl- homocysteine. GNMT is conservative among different animal species. Glycine-N methyltransferase (GNMT) is a potential tumor suppressor that is commonly inactivated in human hepatoma. GNMT is abundant in liver, but very low in HepG2 cells (PMID: 12566990).

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