Validation Data Gallery
Tested Applications
| Positive WB detected in | NK-92 cells, Recombinant protein |
| Positive IHC detected in | human tonsil tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
87707-3-RR targets GNLY/Granulysin in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3381 Product name: Recombinant human Granulysin protein Source: mammalian cells-derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 23-145 aa of NM_006433.5 Sequence: RLSPEYYDLARAHLRDEEKSCPCLAQEGPQGDLLTKTQELGRDYRTCLTIVQKLKKMVDKPTQRSVSNAATRVCRTGRSRWRDVCRNFMRRYQSRVTQGLVAGETAQQICEDLRLCIPSTGPL 相同性解析による交差性が予測される生物種 |
| Full Name | granulysin |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 14-15 kDa |
| GenBank accession number | NM_006433.5 |
| Gene Symbol | GNLY |
| Gene ID (NCBI) | 10578 |
| RRID | AB_3745708 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P22749-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Granulysin is a cationic, saposin-like antimicrobial protein expressed in cytotoxic granules of human CD8+ T cells and NK cells. It exists as 9-kDa (cytolytic) and 15-kDa (secreted) isoforms, killing intracellular pathogens and tumor cells by disrupting membranes or inducing ER stress-mediated apoptosis.(PMID: 17596262;17182542)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GNLY/Granulysin antibody 87707-3-RR | Download protocol |
| WB protocol for GNLY/Granulysin antibody 87707-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |