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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNL3 Monoclonal antibody (67169-1-Ig)

    WB analysis using 67169-1-Ig (same clone as 67169-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67169-1-Ig (GNL3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNL3 Monoclonal antibody (67169-1-Ig)

    IF Staining of HeLa using 67169-1-Ig (same clone as 67169-1-PBS)

    Immunofluorescent analysis of 4% PFA fixed HeLa cells using 67169-1-Ig (GNL3 mouse antibody, green) at dilution of 1:200 and CoraLite488-conjugated Goat Anti-Mouse IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue). This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNL3 Monoclonal antibody (67169-1-Ig)

    IF Staining of HeLa using 67169-1-Ig (same clone as 67169-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 67169-1-Ig (GNL3 antibody) at dilution of 1:100 and CoraLite594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67169-1-PBS in a different storage buffer formulation.

GNL3 Monoclonal antibody, PBS Only

GNL3 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67169-1-PBS
Clone No.3B8F10

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C77032, E2 induced gene 3 protein, E2IG3, GNL3, NNP47, Novel nucleolar protein 47, NS, Nucleostemin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67169-1-PBS targets GNL3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7056

Product name: Recombinant human GNL3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 201-549 aa of BC001024

Sequence: TVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTMLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV

相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein-like 3 (nucleolar)
Calculated molecular weight 62 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC001024
Gene Symbol GNL3
Gene ID (NCBI) 26354
RRIDAB_2882465
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9BVP2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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