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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GNL2 Polyclonal antibody

GNL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10190-2-AP

Synonyms

Autoantigen NGP 1, GNL2, HUMAUANTIG, Ngp 1, NGP1

Validation Data Gallery

Applications:
  • WB analysis using 10190-2-AP

    WB analysis using 10190-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10190-2-AP (GNL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10190-2-AP

    IHC staining of human breast cancer using 10190-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10190-2-AP (GNL2 antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, Ramos cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10190-2-AP targets GNL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNL2 fusion protein Ag0243 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein-like 2 (nucleolar)
Calculated molecular weight 731 aa, 84 kDa
Observed molecular weight84 kDa
GenBank accession numberBC000107
Gene symbol GNL2
Gene ID (NCBI) 29889
RRIDAB_2110075
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GNL2 antibody 10190-2-APDownload protocol
IHC protocol for GNL2 antibody 10190-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols