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Species Reactivity

Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNL1 Polyclonal antibody (14078-1-AP)

    WB analysis using 14078-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using GNL1 Polyclonal antibody (14078-1-AP)

    WB analysis of Jurkat using 14078-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using GNL1 Polyclonal antibody (14078-1-AP)

    WB analysis of Raji using 14078-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using GNL1 Polyclonal antibody (14078-1-AP)

    WB analysis of U-937 using 14078-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using GNL1 Polyclonal antibody (14078-1-AP)

    WB analysis of K-562 using 14078-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14078-1-AP (GNL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using GNL1 Polyclonal antibody (14078-1-AP)

    IHC staining of human heart using 14078-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GNL1 Polyclonal antibody (14078-1-AP)

    IHC staining of human heart using 14078-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using GNL1 Polyclonal antibody (14078-1-AP)

    IHC staining of human skin using 14078-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using GNL1 Polyclonal antibody (14078-1-AP)

    IHC staining of human skin using 14078-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 14078-1-AP (GNL1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GNL1 Polyclonal antibody (14078-1-AP)

    IF Staining of MCF-7 using 14078-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GNL1 antibody (14078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GNL1 Polyclonal antibody (14078-1-AP)

    IF Staining of MCF-7 using 14078-1-AP

    Immunofluorescent analysis of MCF-7 cells, using GNL1 antibody 14078-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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GNL1 Polyclonal antibody

GNL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14078-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HSR1, Guanine nucleotide-binding protein-like 1, GTP-binding protein HSR1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, Raji cells, U-937 cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman heart tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14078-1-AP targets GNL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNL1 fusion protein Ag5203 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein-like 1
Calculated molecular weight 607 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC018366
Gene Symbol GNL1
Gene ID (NCBI) 2794
RRIDAB_10641989
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36915
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Guanine nucleotide-binding protein-like 1 (GNL1), also known as HSR1, belongs to HSR1_MMR1 subfamily of nucleolar GTPases. It encodes 607 amino acids with a molecular mass of 69 kDa and contains basic amino acids rich N-terminus, acidic amino acids rich C-terminus and proline rich-domains. GNL1 plays a critical role in liver cell proliferation, but its function remains largely unknown (PMID: 18255255).

Protocols

Product Specific Protocols
WB protocol for GNL1 antibody 14078-1-APDownload protocol
IHC protocol for GNL1 antibody 14078-1-APDownload protocol
IF protocol for GNL1 antibody 14078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.

Authors - Zewei Tu
View Article