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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse eye tissue using GNGT1 Polyclonal antibody (11884-1-AP)

    WB analysis of mouse eye using 11884-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 11884-1-AP (GNGT1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GNGT1 Polyclonal antibody (11884-1-AP)

    IF Staining of HeLa using 11884-1-AP

    Immunofluorescent analysis of HeLa cells using 11884-1-AP (GNGT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GNGT1 Polyclonal antibody

GNGT1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 11884-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Transducin gamma chain, Guanine nucleotide-binding protein G(T) subunit gamma-T1, GNG1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse eye tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11884-1-AP targets GNGT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2488

Product name: Recombinant human GNGT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-74 aa of BC029367

Sequence: MPVINIEDLTEKDKLKMEVDQLKKEVTLERMLVSKCCEEVRDYVEERSGEDPLVKGIPEDKNPFKELKGGCVIS

相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1
Calculated molecular weight 74 aa, 8 kDa
Observed molecular weight 8 kDa
GenBank accession numberBC029367
Gene Symbol GNGT1
Gene ID (NCBI) 2792
RRIDAB_10858628
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP63211
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

G protein subunit gamma-T1(GNGT1) is encoded by the transducin gamma-subunit gene, which localized on huaman chromosome 7q21.3, participating in various transmembrane signaling systems. Particularly, GNGT1 gene is specific to retinal rod photoreceptors. Present polyclonal anti-GNGT1 antibody (11884-1-AP) is produced by immunizing rabbits with full-length chain of GNGT1 and dectects an 8 kDa band in mouse retinal tissue.

Protocols

Product Specific Protocols
IF protocol for GNGT1 antibody 11884-1-APDownload protocol
WB protocol for GNGT1 antibody 11884-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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