Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, DU 145 cells, HEK-293 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15781-1-AP targets GNG12 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8470 Product name: Recombinant human GNG12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-72 aa of BC005940 Sequence: MSSKTASTNNIAQARRTVQQLRLEASIEGIKVSKASADLMSYCEEHARSDPLLIGIPTSENPFKDKKTCIIL 相同性解析による交差性が予測される生物種 |
| Full Name | guanine nucleotide binding protein (G protein), gamma 12 |
| Calculated molecular weight | 72 aa, 8 kDa |
| Observed molecular weight | 10 kDa |
| GenBank accession number | BC005940 |
| Gene Symbol | GNG12 |
| Gene ID (NCBI) | 55970 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBI6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GNG12 is a G protein γ subunit (chromosome 1p31.3) that regulates heterotrimeric G protein signaling and plays context-dependent roles in cancer, acting as a tumor suppressor in some cancers but pro-tumorigenic in others.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNG12 antibody 15781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |