Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 16090-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16090-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16090-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16090-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16090-1-AP (GNB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 16090-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 16090-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 16090-1-AP (GNB2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 16090-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GNB2 antibody (16090-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of HepG2 using 16090-1-AP
Immunofluorescent analysis of HepG2 cells, using GNB2 antibody 16090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

GNB2 Polyclonal antibody

GNB2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16090-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, G protein subunit beta-2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, Jurkat cells
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

16090-1-AP targets GNB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9125 Product name: Recombinant human GNB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-340 aa of BC010073 Sequence: MSELEQLRQEAEQLRNQIRDARKACGDSTLTQITAGLDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNFVACGGLDNICSIYSLKTREGNVRVSRELPGHTGYLSCCRFLDDNQIITSSGDTTCALWDIETGQQTVGFAGHSGDVMSLSLAPDGRTFVSGACDASIKLWDVRDSMCRQTFIGHESDINAVAFFPNGYAFTTGSDDATCRLFDLRADQELLMYSHDNIICGITSVAFSRSGRLLLAGYDDFNCNIWDAMKGDRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN 相同性解析による交差性が予測される生物種
Full Name	guanine nucleotide binding protein (G protein), beta polypeptide 2
Calculated molecular weight	340 aa, 37 kDa
Observed molecular weight	37 kDa
GenBank accession number	BC010073
Gene Symbol	GNB2
Gene ID (NCBI)	2783
RRID	AB_2111939
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P62879
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GNB2(Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2) is also named as G protein subunit beta-2, transducin beta chain 2 and belongs to the WD repeat G protein beta family. G proteins are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Protocols

Product Specific Protocols
IF protocol for GNB2 antibody 16090-1-AP	Download protocol
IHC protocol for GNB2 antibody 16090-1-AP	Download protocol
WB protocol for GNB2 antibody 16090-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse		Front Cardiovasc Med Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis. Authors - Yue Zheng View Article
human,mouse	WB	PLoS Biol KCTD proteins regulate morphine dependence via heterologous sensitization of adenylyl cyclase 1 in mice Authors - Zhong Ding View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 16090-1-AP

WB analysis of HeLa using 16090-1-AP

WB analysis of HEK-293 using 16090-1-AP

WB analysis of Jurkat using 16090-1-AP

IHC staining of human ovary tumor using 16090-1-AP