Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12758-1-AP targets GNAZ in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3255 Product name: Recombinant human GNAZ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC026342 Sequence: MGCRQSSEEKEAARRSRRIDRHLRSESQRQRREIKLLLLGTSNSGKSTIVKQMKIIHSGGFNLEACKEYKPLIIYNAIDSLTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRRLWADQGAQACFSRSSEYHLEDNAAYYLNDLERIAAADYIPTVEDILRSRDMTTGIVENKFTFKELTFKMVDVGGQRSERKKWIHCFEGVTAIIFCVELSGYDLKLYEDNQTSRMAESLRLFDSICNNNWFINTSLILFLNKKDLLAEKIRRIPLTICFPEYKGQNTYEEAAVYIQRQFEDLNRNKETKEIYSHFTCATDTSNIQFVFDAVTDVIIQNNLKYIGLC 相同性解析による交差性が予測される生物種 |
| Full Name | guanine nucleotide binding protein (G protein), alpha z polypeptide |
| Calculated molecular weight | 355 aa, 41 kDa |
| Observed molecular weight | 38-41 kDa |
| GenBank accession number | BC026342 |
| Gene Symbol | GNAZ |
| Gene ID (NCBI) | 2781 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P19086 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Guanine nucleotide-binding protein G(z) subunit alpha(GNAZ) is a member of a G protein subfamily that mediates signal transduction in pertussis toxin-insensitive systems. Heterotrimeric G proteins consist of alpha (α), beta (β), and gamma (γ) subunits. G proteins act as molecular switches inside cells, functioning to relay and transmit signals from cell surface receptors to intracellular effectors(PMID: 35052777). GNAS transduces signals from G protein-coupled receptors (GPCR) to adenyl cyclase (AC), and finally regulates the expression of cyclic adenosine monophosphate (cAMP)(PMID: 32700107).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNAZ antibody 12758-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |