Various lysates were subjected to SDS PAGE followed by western blot with 55167-1-AP (GNAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

WB results of GNAT1 antibody (55167-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

mouse eye tissue were subjected to SDS PAGE followed by western blot with 55167-1-AP (GNAT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 55167-1-AP (GNAT1 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 55167-1-AP (GNAT1 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 55167-1-AP (GNAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GNAT1 (55167-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 55167-1-PBS in a different storage buffer formulation.