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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis using 68191-1-Ig (same clone as 68191-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis of MCF-7 using 68191-1-Ig (same clone as 68191-1-PBS)

    WB result of GNAQ antibody (68191-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNAQ transfected MCF-7 cells. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig cerebellum tissue using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis of pig cerebellum using 68191-1-Ig (same clone as 68191-1-PBS)

    pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis using 68191-1-Ig (same clone as 68191-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat cerebellum tissue using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis of rat cerebellum using 68191-1-Ig (same clone as 68191-1-PBS)

    rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GNAQ Monoclonal antibody (68191-1-Ig)

    WB analysis of HeLa using 68191-1-Ig (same clone as 68191-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68191-1-Ig (GNAQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68191-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GNAQ Monoclonal antibody, PBS Only

GNAQ Monoclonal Antibody for WB, ELISA
Cat No. 68191-1-PBS
Clone No.2H5G6

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Synonyms

2H5G6, CMC1, G ALPHA q, GAQ, Guanine nucleotide-binding protein alpha-q

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68191-1-PBS targets GNAQ in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26178

Product name: Recombinant human GNAQ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 84-205 aa of BC057777

Sequence: FTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPTTGIIEYPFDLQSVIFRMVD

相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), q polypeptide
Calculated molecular weight 42 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC057777
Gene Symbol GNAQ
Gene ID (NCBI) 2776
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP50148
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GNAQ is a proto-oncogene closely related to the α subunit of guanine nucleotide-binding proteins (G proteins) that serve as key intermediates between membrane-bound G-protein-coupled receptors (GPCRs) and GPCR signalling nodes. Aberrant expression and activity of G proteins and GPCRs are frequently associated with tumorigenesis. Recent studies show that oncogenic activating mutations in GNAQ are present in 80% of the melanomas. The calculated molecular weight of GNAQ is 42 kDa. This antibody can detect a band of 38-40 kDa in SDS-PAGE. (PMID: 33993733)

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