Validation Data Gallery
Tested Applications
| Positive WB detected in | fetal human brain tissue, mouse brain tissue, SKOV-3 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue, human kidney tissue, human lung cancer tissue, mouse ovary tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
11136-1-AP targets GNAI2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1597 Product name: Recombinant human GNAI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC012138 Sequence: MGCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEEQGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF 相同性解析による交差性が予測される生物種 |
| Full Name | guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2 |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC012138 |
| Gene Symbol | GNAI2 |
| Gene ID (NCBI) | 2771 |
| RRID | AB_2232442 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04899 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GNAI2, also named as GNAI2B, belongs to the G-alpha family. G(i/o/t/z) subfamily. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. GNAI2 is 93% homolog to GNAI1, 94% to GNAI3, 85% to GNAT3, 82% to GNAT2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GNAI2 antibody 11136-1-AP | Download protocol |
| IHC protocol for GNAI2 antibody 11136-1-AP | Download protocol |
| IF protocol for GNAI2 antibody 11136-1-AP | Download protocol |
| IP protocol for GNAI2 antibody 11136-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis | ||
Cells Finerenone Alleviates Over-Activation of Complement C5a-C5aR1 Axis of Macrophages by Regulating G Protein Subunit Alpha i2 to Improve Diabetic Nephropathy | ||
bioRxiv Inhibitory G proteins play multiple roles to polarize sensory hair cell morphogenesis | ||
Adv Sci (Weinh) Targeting Squalene Epoxidase Confers Metabolic Vulnerability and Overcomes Chemoresistance in HNSCC |