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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNA15 Polyclonal antibody (12078-1-AP)

    WB analysis using 12078-1-AP

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 12078-1-AP (GNA15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GNA15 Polyclonal antibody (12078-1-AP)

    WB analysis using 12078-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12078-1-AP (GNA15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GNA15 Polyclonal antibody (12078-1-AP)

    IHC staining of human small intestine using 12078-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12078-1-AP (GNA15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using GNA15 Polyclonal antibody (12078-1-AP)

    IHC staining of human small intestine using 12078-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 12078-1-AP (GNA15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GNA15 Polyclonal antibody

GNA15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12078-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

G alpha 15, G alpha 16, G protein subunit alpha 15, G protein subunit alpha 16, GNA15, GNA16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMOLT-4 cells, mouse spleen tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12078-1-AP targets GNA15 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GNA15 fusion protein Ag2738 相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), alpha 15 (Gq class)
Calculated molecular weight 374 aa, 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC013585
Gene Symbol GNA15
Gene ID (NCBI) 2769
RRIDAB_3085389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30679
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GNA15, also known as GNA16, belongs to the G-alpha family and G(q) subfamily. The protein encoded by the GNA15 gene is the alpha subunit of the G protein, which participates in the regulation of cell proliferation and apoptosis. GNA15 is highly expressed in some cell types, such as hematopoietic stem cells and epithelial cells during specific stages of differentiation. GNA15 is known to play as an oncogene in several tumors, such as gastroenteropancreatic neuroendocrine neoplasia (GEP-NEN), liver cancer, pancreatic ductal adenocarcinoma and ovarian cancer (PMID: 34552875). The molecular weight of GNA15 is 44 kDa.

Protocols

Product Specific Protocols
WB protocol for GNA15 antibody 12078-1-APDownload protocol
IHC protocol for GNA15 antibody 12078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols