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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GNA13 Monoclonal antibody (67188-1-Ig)

    WB analysis using 67188-1-Ig (same clone as 67188-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67188-1-Ig (GNA13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GNA13 Monoclonal antibody (67188-1-Ig)

    WB analysis of HEK-293 using 67188-1-Ig (same clone as 67188-1-PBS)

    WB result of GNA13 antibody (67188-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GNA13 transfected HEK-293 cells. This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GNA13 Monoclonal antibody (67188-1-Ig)

    IHC staining of human breast cancer using 67188-1-Ig (same clone as 67188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GNA13 Monoclonal antibody (67188-1-Ig)

    IHC staining of human breast cancer using 67188-1-Ig (same clone as 67188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNA13 Monoclonal antibody (67188-1-Ig)

    IHC staining of human liver cancer using 67188-1-Ig (same clone as 67188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GNA13 Monoclonal antibody (67188-1-Ig)

    IHC staining of human liver cancer using 67188-1-Ig (same clone as 67188-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67188-1-Ig (GNA13 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using GNA13 Monoclonal antibody (67188-1-Ig)

    IF Staining of SH-SY5Y using 67188-1-Ig (same clone as 67188-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using GNA13 antibody (67188-1-Ig, Clone: 2G8F10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67188-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GNA13 Monoclonal antibody, PBS Only

GNA13 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67188-1-PBS
Clone No.2G8F10

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

G alpha 13, G alpha-13, G protein subunit alpha 13, G13, G-protein subunit alpha-13

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67188-1-PBS targets GNA13 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7316

Product name: Recombinant human GNA13 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-377 aa of BC036756

Sequence: MADFLPSRSVLSVCFPGCLLTSGEAEQQRKSKEIDKCLSREKTYVKRLVKILLLGAGESGKSTFLKQMRIIHGQDFDQRAREEFRPTIYSNVIKGMRVLVDAREKLHIPWGDNSNQQHGDKMMSFDTRAPMAAQGMVETRVFLQYLPAIRALWADSGIQNAYDRRREFQLGESVKYFLDNLDKLGEPDYIPSQQDILLARRPTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVSSSEFDQVLMEDRLTNRLTESLNIFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLVECFRNKRRDQQQKPLYHHFTTAINTENIRLVFRDVKDTILHDNLKQLMLQ

相同性解析による交差性が予測される生物種
Full Name guanine nucleotide binding protein (G protein), alpha 13
Calculated molecular weight 44 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC036756
Gene Symbol GNA13
Gene ID (NCBI) 10672
RRIDAB_2882483
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14344
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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