Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 16376-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 16376-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 16376-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16376-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 16376-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 16376-1-AP (GMPS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16376-1-AP
IP result of anti-GMPS (IP:16376-1-AP, 4ug; Detection:16376-1-AP 1:800) with HeLa cells lysate 1080ug.
IHC staining of human liver cancer using 16376-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16376-1-AP (GMPS antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver cancer using 16376-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16376-1-AP (GMPS antibody) at dilution of 1:100 (under 40x lens).

GMPS Polyclonal antibody

GMPS Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 16376-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Glutamine amidotransferase, GMP synthetase, GMPS

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HL-60 cells, Jurkat cells, mouse brain tissue, mouse testis tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

16376-1-AP targets GMPS in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9350 Product name: Recombinant human GMPS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 345-693 aa of BC012178 Sequence: VKIANEVIGEMNLKPEEVFLAQGTLRPDLIESASLVASGKAELIKTHHNDTELIRKLREEGKVIEPLKDFHKDEVRILGRELGLPEELVSRHPFPGPGLAIRVICAEEPYICKDFPETNNILKIVADFSASVKKPHTLLQRVKACTTEEDQEKLMQITSLHSLNAFLLPIKTVGVQGDCRSYSYVCGISSKDEPDWESLIFLARLIPRMCHNVNRVVYIFGPPVKEPPTDVTPTFLTTGVLSTLRQADFEAHNILRESGYAGKISQMPVILTPLHFDRDPLQKQPSCQRSVVIRTFITSDFMTGIPATPGNEIPVEVVLKMVTEIKKIPGISRIMYDLTSKPPGTTEWE 相同性解析による交差性が予測される生物種
Full Name	guanine monphosphate synthetase
Calculated molecular weight	693 aa, 77 kDa
Observed molecular weight	67 kDa
GenBank accession number	BC012178
Gene Symbol	GMPS
Gene ID (NCBI)	8833
RRID	AB_2232412
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49915
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for GMPS antibody 16376-1-AP	Download protocol
IP protocol for GMPS antibody 16376-1-AP	Download protocol
WB protocol for GMPS antibody 16376-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB	Cell De novo and salvage purine synthesis pathways across tissues and tumors Authors - Diem H Tran View Article
mouse	WB	Nat Immunol Purine salvage pathway protects CD8(+) T cells from metabolic stress. Authors - Masaki Tajima View Article
human	WB	Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. Authors - Eunus S Ali View Article
		Mol Cell Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle. Authors - Mushtaq A. Nengroo View Article
human	WB,IHC	J Biomed Sci TRIM21-SERPINB5 aids GMPS repression to protect nasopharyngeal carcinoma cells from radiation-induced apoptosis. Authors - Panpan Zhang View Article
mouse	WB	Cell Rep Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy. Authors - Nicole M Sayles View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Validation Data Gallery

WB analysis of HeLa using 16376-1-AP

WB analysis of mouse testis using 16376-1-AP

WB analysis of HL-60 using 16376-1-AP

WB analysis of mouse brain using 16376-1-AP

WB analysis of Jurkat using 16376-1-AP

IP experiment of HeLa using 16376-1-AP

IHC staining of human liver cancer using 16376-1-AP

IHC staining of human liver cancer using 16376-1-AP

GMPS Polyclonal antibody

GMPS Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

De novo and salvage purine synthesis pathways across tissues and tumors

Purine salvage pathway protects CD8(+) T cells from metabolic stress.

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle.

TRIM21-SERPINB5 aids GMPS repression to protect nasopharyngeal carcinoma cells from radiation-induced apoptosis.

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

Able^™

Validation Data Gallery

WB analysis of HeLa using 16376-1-AP

WB analysis of mouse testis using 16376-1-AP

WB analysis of HL-60 using 16376-1-AP

WB analysis of mouse brain using 16376-1-AP

WB analysis of Jurkat using 16376-1-AP

IP experiment of HeLa using 16376-1-AP

IHC staining of human liver cancer using 16376-1-AP

IHC staining of human liver cancer using 16376-1-AP

GMPS Polyclonal antibody

GMPS Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

De novo and salvage purine synthesis pathways across tissues and tumors

Purine salvage pathway protects CD8(+) T cells from metabolic stress.

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle.

TRIM21-SERPINB5 aids GMPS repression to protect nasopharyngeal carcinoma cells from radiation-induced apoptosis.

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

Able™

Able^™