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Host
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GMPPA Polyclonal antibody

GMPPA Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 15517-1-AP

Synonyms

GMPPA

Validation Data Gallery

Applications:
  • WB analysis using 15517-1-AP

    WB analysis using 15517-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15517-1-AP (GMPPA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 15517-1-AP

    IP experiment of HEK-293 using 15517-1-AP

    IP result of anti-GMPPA (IP:15517-1-AP, 4ug; Detection:15517-1-AP 1:500) with HEK-293 cells lysate 2600ug.

  • IF Staining of HeLa using 15517-1-AP

    IF Staining of HeLa using 15517-1-AP

    Immunofluorescent analysis of Hela cells, using GMPPA antibody 15517-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15517-1-AP targets GMPPA in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMPPA fusion protein Ag7789 相同性解析による交差性が予測される生物種
Full Name GDP-mannose pyrophosphorylase A
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC007456
Gene symbol GMPPA
Gene ID (NCBI) 29926
RRIDAB_2111056
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GMPPA antibody 15517-1-APDownload protocol
IF protocol for GMPPA antibody 15517-1-APDownload protocol
IP protocol for GMPPA antibody 15517-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

A Trace Amount of Galactose, a Major Component of Milk Sugar, Allows Maturation of Glycoproteins during Sugar Starvation.

Authors - Norio Sasaoka
View Article
mouseWB

Front Mol Biosci

Altered Glycosylation in the Aging Heart.

Authors - Patricia Franzka
View Article
human,mouseWB

Front Nutr

Altered mannose metabolism in chronic stress and depression is rapidly reversed by vitamin B12

Authors - Patricia Franzka
View Article
mouseWB

Biomedicines

Impact of Hypermannosylation on the Structure and Functionality of the ER and the Golgi Complex

Authors - Patricia Franzka
  • KO Validated
View Article