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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GMIP Polyclonal antibody

GMIP Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 25917-1-AP

Synonyms

GEM interacting protein, GMIP

Validation Data Gallery

Applications:
  • WB analysis using 25917-1-AP

    WB analysis using 25917-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25917-1-AP (GMIP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 25917-1-AP

    IP experiment of Jurkat using 25917-1-AP

    IP result of anti-GMIP (IP:25917-1-AP, 4ug; Detection:25917-1-AP 1:500) with Jurkat cells lysate 4000ug.

  • IF Staining of HepG2 using 25917-1-AP

    IF Staining of HepG2 using 25917-1-AP

    Immunofluorescent analysis of HepG2 cells using 25917-1-AP (GMIP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HepG2 using 25917-1-AP

    IF Staining of HepG2 using 25917-1-AP

    Immunofluorescent analysis of HepG2 cells using 25917-1-AP (GMIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IP detected inJurkat cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

25917-1-AP targets GMIP in WB, IP, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMIP fusion protein Ag23239 相同性解析による交差性が予測される生物種
Full Name GEM interacting protein
Observed molecular weight 130 kDa
GenBank accession numberBC126436
Gene symbol GMIP
Gene ID (NCBI) 51291
RRIDAB_2880295
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo.

Protocols

Product Specific Protocols
WB protocol for GMIP antibody 25917-1-APDownload protocol
IF protocol for GMIP antibody 25917-1-APDownload protocol
IP protocol for GMIP antibody 25917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols