Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of rat brain using 13625-1-AP (same clone as 13625-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 13625-1-AP (same clone as 13625-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
WB analysis of mouse placenta using 13625-1-AP (same clone as 13625-1-PBS)
mouse placenta tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 13625-1-AP (same clone as 13625-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 13625-1-AP (GMFG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IP experiment of mouse spleen using 13625-1-AP (same clone as 13625-1-PBS)
IP result of anti-GMFG (IP:13625-1-AP, 4ug; Detection:13625-1-AP 1:600) with mouse spleen tissue lysate 2000 ug. This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13625-1-AP (GMFG antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13625-1-AP (GMFG antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human spleen using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human spleen using 13625-1-AP (GMFG antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13625-1-AP (GMFG Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 13625-1-AP (same clone as 13625-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13625-1-AP (GMFG Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13625-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

GMFG Polyclonal antibody, PBS Only

GMFG Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 13625-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Glia maturation factor gamma, glia maturation factor, gamma, GMF GAMMA, GMF-gamma

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13625-1-PBS targets GMFG in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4538 Product name: Recombinant human GMFG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-141 aa of BC032819 Sequence: SDSLVVCEVDPELTEKLRKFRFRKETDNAAIIMKVDKDRQMVVLEEEFQNISPEELKMELPERQPRFVVYSYKYVHDDGRVSYPLCFIFSSPVGCKPEQQMMYAGSKNRLVQTAELTKVFEIRTTDDLTEAWLQEKLSFFR 相同性解析による交差性が予測される生物種
Full Name	glia maturation factor, gamma
Calculated molecular weight	141 aa, 17 kDa
Observed molecular weight	17 kDa
GenBank accession number	BC032819
Gene Symbol	GMFG
Gene ID (NCBI)	9535
RRID	AB_2110929
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60234
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

GMFG, is a highly conserved brain-specific protein that belongs to the GMF subfamily of the larger Actin-binding protein ADF family. GMFG is preferentially expressed in blood vessel cells and immune cells and its ectopic expression enhances cellular functions such as tube-formation and migration in vitro. GMFG mediates neutrophil and T-lymphocyte migration via regulation of actin cytoskeletal reorganization. GMFG also acts as an intracellular regulator of stress-activated signal transduction by demonstrating activation of p38 MAP kinase and transcription factor NF-kB in astrocytes.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of rat brain using 13625-1-AP (same clone as 13625-1-PBS)

WB analysis of human placenta using 13625-1-AP (same clone as 13625-1-PBS)

WB analysis of mouse placenta using 13625-1-AP (same clone as 13625-1-PBS)

WB analysis of human heart using 13625-1-AP (same clone as 13625-1-PBS)

IP experiment of mouse spleen using 13625-1-AP (same clone as 13625-1-PBS)

IHC staining of human lung cancer using 13625-1-AP (same clone as 13625-1-PBS)

IHC staining of human lung cancer using 13625-1-AP (same clone as 13625-1-PBS)

IHC staining of human kidney using 13625-1-AP (same clone as 13625-1-PBS)

IHC staining of human kidney using 13625-1-AP (same clone as 13625-1-PBS)

IHC staining of human placenta using 13625-1-AP (same clone as 13625-1-PBS)

IHC staining of human placenta using 13625-1-AP (same clone as 13625-1-PBS)

IHC staining of human testis using 13625-1-AP (same clone as 13625-1-PBS)