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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using GMEB2 Polyclonal antibody (13314-1-AP)

    WB analysis of human placenta using 13314-1-AP (same clone as 13314-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using GMEB2 Polyclonal antibody (13314-1-AP)

    WB analysis of mouse thymus using 13314-1-AP (same clone as 13314-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using GMEB2 Polyclonal antibody (13314-1-AP)

    WB analysis of mouse ovary using 13314-1-AP (same clone as 13314-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13314-1-AP (GMEB2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GMEB2 Polyclonal antibody (13314-1-AP)

    IHC staining of human cervical cancer using 13314-1-AP (same clone as 13314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using GMEB2 Polyclonal antibody (13314-1-AP)

    IHC staining of human cervical cancer using 13314-1-AP (same clone as 13314-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13314-1-AP (GMEB2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GMEB2 Polyclonal antibody (13314-1-AP)

    IF Staining of MCF-7 using 13314-1-AP (same clone as 13314-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GMEB2 antibody (13314-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13314-1-PBS in a different storage buffer formulation.

GMEB2 Polyclonal antibody, PBS Only

GMEB2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13314-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

P79PIF, KIAA1269, GMEB-2, GMEB 2, Glucocorticoid modulatory element-binding protein 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13314-1-PBS targets GMEB2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4157

Product name: Recombinant human GMEB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-304 aa of BC036305

Sequence: MATPDVSVHMEEVVVVTTPDTAVDGSGVEGVKTVLVTTNLAPHGGDLTEDNMETENAAAAAAAAFTASSQLKEAVLVKMAEEGENLEAEIVYPITCGDSRANLIWRKFVCPGINVKCVQYDEHVISPKEFVHLAGKSTLKDWKRAIRMNGIMLRKIMDSGELDFYQHDKVCSNTCRSTKIDLSGARVSLSSPTSAEYIPLTPAAADVNGSPATITIETCEDPGDWTAAIGDDTFTFWRGLKDAGLLDEVIQEFHQELVETMRGLQQRVQDPPLQLRDAVLLNNIVQNFGMLDLVKKVLASHKCQ

相同性解析による交差性が予測される生物種
Full Name glucocorticoid modulatory element binding protein 2
Calculated molecular weight 530 aa, 56 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC036305
Gene Symbol GMEB2
Gene ID (NCBI) 26205
RRIDAB_2110829
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKD1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GMEB2, also named as Glucocorticoid modulatory element-binding protein 2, is a 530 amino acid protein, which is expressed in peripheral blood lymphocytes and fetal liver. GMEB2 as a trans-acting factor that binds to glucocorticoid modulatory elements (GME) present in the TAT (tyrosine aminotransferase) promoter and increases sensitivity to low concentrations of glucocorticoids. The calcualted molecular weight of GMEB2 is 56 kDa, but modified GMEB2 is about 65-70 kDa.

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