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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GMEB1-Specific Recombinant antibody (84448-1-RR)

    WB analysis using 84448-1-RR (same clone as 84448-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84448-1-RR (GMEB1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84448-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using GMEB1-Specific Recombinant antibody (84448-1-RR)

    FC experiment of HeLa using 84448-1-RR (same clone as 84448-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GMEB1-Specific Recombinant antibody (84448-1-RR, Clone:241447C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84448-1-PBS in a different storage buffer formulation.

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GMEB1-Specific Recombinant antibody, PBS Only

GMEB1-Specific Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84448-1-PBS
Clone No.241447C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

GMEB1, GMEB-1, GMEB 1, Glucocorticoid modulatory element-binding protein 1, DNA-binding protein p96PIF

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84448-1-PBS targets GMEB1-Specific in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name glucocorticoid modulatory element binding protein 1
Calculated molecular weight 63 kDa
Observed molecular weight88 kDa
GenBank accession numberNM_006582
Gene Symbol GMEB1
Gene ID (NCBI) 10691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y692
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

GMEB1 is a trans-acting factor that binds to glucocorticoid modulatory elements (GME), acting in estrogen receptor-regulated pathway GREB1 is expressed in proliferating prostate tissue & prostate cancer and is thought to function in hormone-dependent cancer growth. It associates with GMEB2 protein, and the complex is essential for parvovirus DNA replication.