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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GMCL1 Polyclonal antibody (15575-1-AP)

    WB analysis using 15575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15575-1-AP (GMCL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human thyroid tissue using GMCL1 Polyclonal antibody (15575-1-AP)

    IHC staining of human thyroid using 15575-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human thyroid tissue using GMCL1 Polyclonal antibody (15575-1-AP)

    IHC staining of human thyroid using 15575-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using GMCL1 Polyclonal antibody (15575-1-AP)

    IHC staining of human testis using 15575-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human testis tissue using GMCL1 Polyclonal antibody (15575-1-AP)

    IHC staining of human testis using 15575-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15575-1-AP (GMCL1 Antibody) at dilution of 1:50.

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GMCL1 Polyclonal antibody

GMCL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15575-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

SPATA29, Germ cell-less protein-like 1, Germ cell less protein like 1, GCL1, GCL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inhuman thyroid tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15575-1-AP targets GMCL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GMCL1 fusion protein Ag7929 相同性解析による交差性が予測される生物種
Full Name germ cell-less homolog 1 (Drosophila)
Calculated molecular weight 59 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC007420
Gene Symbol GMCL1
Gene ID (NCBI) 64395
RRIDAB_2878153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96IK5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GMCL1, also named as GCL, functions in spermatogenesis. It enhances the degradation of MDM2 and increases the amount of p53 probably by modulating the nucleocytoplasmic transport. In WB test, the antibody recognizes 55 kDa band in our detection.

Protocols

Product Specific Protocols
WB protocol for GMCL1 antibody 15575-1-APDownload protocol
IHC protocol for GMCL1 antibody 15575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols