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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GM2A Monoclonal antibody (66080-1-Ig)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    WB result of GM2A antibody (66080-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GM2A transfected HeLa cells. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GM2A Monoclonal antibody (66080-1-Ig)

    WB analysis of HeLa using 66080-1-Ig (same clone as 66080-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using GM2A Monoclonal antibody (66080-1-Ig)

    WB analysis of human placenta using 66080-1-Ig (same clone as 66080-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66080-1-Ig (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using GM2A Monoclonal antibody (66080-1-Ig)

    FC experiment of HEK-293 using 66080-1-Ig (same clone as 66080-1-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (66080-1-Ig, Clone:1E4D3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66080-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GM2A Monoclonal antibody, PBS Only

GM2A Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66080-1-PBS
Clone No.1E4D3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

1E4D3, Ganglioside GM2 activator isoform short, GM2AP, GM2-AP, SAP3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66080-1-PBS targets GM2A in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4394

Product name: Recombinant human GM2A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-193 aa of BC009273

Sequence: MQSLMQAPLLIALGLLLAAPAQAHLKKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIVVPGNVTLSVVGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI

相同性解析による交差性が予測される生物種
Full Name GM2 ganglioside activator
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009273
Gene Symbol GM2A
Gene ID (NCBI) 2760
RRIDAB_11182593
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP17900
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

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