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Validation Data Gallery

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  • Western Blot (WB) analysis of rat kidney tissue using GM2A Polyclonal antibody (10864-2-AP)

    WB analysis of rat kidney using 10864-2-AP (same clone as 10864-2-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 10864-2-AP (GM2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using GM2A Polyclonal antibody (10864-2-AP)

    WB analysis of mouse kidney using 10864-2-AP (same clone as 10864-2-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10864-2-AP (GM2A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using GM2A Polyclonal antibody (10864-2-AP)

    IP experiment of HEK-293 using 10864-2-AP (same clone as 10864-2-PBS)

    IP result of anti-GM2A (IP:10864-2-AP, 3ug; Detection:10864-2-AP 1:600) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using GM2A Polyclonal antibody (10864-2-AP)

    IHC staining of human placenta using 10864-2-AP (same clone as 10864-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using GM2A Polyclonal antibody (10864-2-AP)

    IHC staining of human placenta using 10864-2-AP (same clone as 10864-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10864-2-AP (GM2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HEK-293 cells using GM2A Polyclonal antibody (10864-2-AP)

    FC experiment of HEK-293 using 10864-2-AP (same clone as 10864-2-PBS)

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human GM2A (10864-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10864-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GM2A Polyclonal antibody, PBS Only

GM2A Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA
Cat No. 10864-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10864-2-PBS targets GM2A in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1295

Product name: Recombinant human GM2A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-193 aa of BC009273

Sequence: MQSLMQAPLLIALGLLLAAPAQAHLKKPSQLSSFSWDNCDEGKDPAVIRSLTLEPDPIVVPGNVTLSVVGSTSVPLSSPLKVDLVLEKEVAGLWIKIPCTDYIGSCTFEHFCDVLDMLIPTGEPCPEPLRTYGLPCHCPFKEGTYSLPKSEFVVPDLELPSWLTTGNYRIESVLSSSGKRLGCIKIAASLKGI

相同性解析による交差性が予測される生物種
Full Name GM2 ganglioside activator
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC009273
Gene Symbol GM2A
Gene ID (NCBI) 2760
RRIDAB_2110807
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP17900
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GM2A, also named as GM2-AP and SAP-3, is the large binding pocket which can accommodate several single chain phospholipids and fatty acids. GM2A also exhibits some calcium-independent phospholipase activity. It plays a key role in the degradation of ganglioside GM2 (GM2). GM2A stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A.

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