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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GLS2 Polyclonal antibody

GLS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20171-1-AP

Synonyms

GA, GLS, GLS2, hLGA, L glutaminase, L glutamine amidohydrolase, LGA

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 20171-1-AP

    WB analysis of HeLa using 20171-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20171-1-AP (GLS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 20171-1-AP

    IHC staining of human liver using 20171-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20171-1-AP (GLS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 20171-1-AP

    IHC staining of human liver using 20171-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20171-1-AP (GLS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20171-1-AP targets GLS2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GLS2 fusion protein Ag14003 相同性解析による交差性が予測される生物種
Full Name glutaminase 2 (liver, mitochondrial)
Calculated molecular weight 602 aa, 66 kDa
Observed molecular weight66-70 kDa
GenBank accession numberBC059370
Gene symbol GLS2
Gene ID (NCBI) 27165
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLS2, also named as GA and GLS, belongs to the glutaminase family. It is Glutaminase liver isoform . GLS2 plays an important role in the regulation of glutamine catabolism. It promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. GLS2 may play a role in preventing tumor proliferation. This antibody is specific to GLS2.

Protocols

Product Specific Protocols
WB protocol for GLS2 antibody 20171-1-APDownload protocol
IHC protocol for GLS2 antibody 20171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols