Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 23549-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:600 incubated at 4 degree celsius over night.
WB analysis of HeLa using 23549-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23549-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 23549-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 23549-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 23549-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 23549-1-AP
IP result of anti-KGA/GAM/GAC (IP:23549-1-AP, 4ug; Detection:23549-1-AP 1:1000) with HEK-293 cells lysate 1200ug.
IHC staining of human kidney using 23549-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.
IHC staining of human kidney using 23549-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.
IHC staining of human brain using 23549-1-AP
Immunohistochemical analysis of paraffin-embedded human brain slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.
IHC staining of human brain using 23549-1-AP
Immunohistochemical analysis of paraffin-embedded human brain slide using 23549-1-AP (KGA/GAM/GAC antibody at dilution of 1:50.
IF Staining of HEK-293 using 23549-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 23549-1-AP (KGA/GAM/GAC antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 23549-1-AP
Immunofluorescent analysis of HeLa cells using 23549-1-AP (KGA/GAM/GAC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Featured Product
KD/KO Validated

KGA/GAM/GAC Polyclonal antibody

KGA/GAM/GAC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 23549-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

AAD20, GAC, GAM, GLS, GLS1, glutaminase, K glutaminase, KGA, KGA/GAM/GAC, KIAA0838, L glutamine amidohydrolase

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human kidney tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

23549-1-AP targets KGA/GAM/GAC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20354 Product name: Recombinant human GLS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC038507 Sequence: MMRLRGSGMLRDLLLRSPAGVSATLRRAQPLVTLCRRPRGGGRPAAGPAAAARLHPWWGGGGWPAEPLARGLSSSPSEILQELGKGSTHPQPGVSPPAAPAAPGPKDGPGETDAFGNSEGKELVASGENKIKQGLLPSLE 相同性解析による交差性が予測される生物種
Full Name	glutaminase
Calculated molecular weight	669 aa, 73 kDa
Observed molecular weight	58 kDa, 65 kDa, 83 kDa
GenBank accession number	BC038507
Gene Symbol	GLS
Gene ID (NCBI)	2744
RRID	AB_2879295
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O94925
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa(PMID:12408749). Ping Gao et al. (2009) determined that mitochondrial glutaminase expression (GLS, molecular mass of ~58 kDa) is increased ~10-fold in response to Myc. It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). GLS has 3 isoforms produced by alternative splicing and this antibody can recognize all the 3 isoforms(KGA,GAM,GAC) of GLS.

Protocols

Product Specific Protocols
WB protocol for KGA/GAM/GAC antibody 23549-1-AP	Download protocol
IHC protocol for KGA/GAM/GAC antibody 23549-1-AP	Download protocol
IF protocol for KGA/GAM/GAC antibody 23549-1-AP	Download protocol
IP protocol for KGA/GAM/GAC antibody 23549-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis. Authors - Bin Jiang KO Validated View Article
human	WB	Redox Biol Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels. Authors - M Ryan Smith View Article
human	IHC,WB	Biosci Rep Progesterone inhibits endometrial cancer growth by inhibiting glutamine metabolism through ASCT2 Authors - Jinqiu Guo View Article
human	IF	Adv Sci (Weinh) Overcoming Cancer Persister Cells by Stabilizing the ATF4 Promoter G-quadruplex Authors - Chengmei Xiao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 23549-1-AP

WB analysis of HeLa using 23549-1-AP

WB analysis of HEK-293 using 23549-1-AP

WB analysis of mouse brain using 23549-1-AP

WB analysis of mouse liver using 23549-1-AP

WB analysis of rat brain using 23549-1-AP

IP experiment of HEK-293 using 23549-1-AP

IHC staining of human kidney using 23549-1-AP

IHC staining of human kidney using 23549-1-AP

IHC staining of human brain using 23549-1-AP

IHC staining of human brain using 23549-1-AP

IF Staining of HEK-293 using 23549-1-AP

IF Staining of HeLa using 23549-1-AP