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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLRX3 Polyclonal antibody (11254-1-AP)

    WB analysis using 11254-1-AP (same clone as 11254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11254-1-AP (GLRX3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11254-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GLRX3 Polyclonal antibody (11254-1-AP)

    WB analysis using 11254-1-AP (same clone as 11254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11254-1-AP (GLRX3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GLRX3 Polyclonal antibody (11254-1-AP)

    IHC staining of human stomach cancer using 11254-1-AP (same clone as 11254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11254-1-AP (GLRX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GLRX3 Polyclonal antibody (11254-1-AP)

    IHC staining of human stomach cancer using 11254-1-AP (same clone as 11254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11254-1-AP (GLRX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11254-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GLRX3 Polyclonal antibody (11254-1-AP)

    IHC staining of human colon cancer using 11254-1-AP (same clone as 11254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11254-1-AP (GLRX3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11254-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GLRX3 Polyclonal antibody, PBS Only

GLRX3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11254-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Glutaredoxin 3, GLRX4, Glutaredoxin-3, GRX3, GRX4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11254-1-PBS targets GLRX3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1775

Product name: Recombinant human GLRX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-289 aa of BC014372

Sequence: AQMNEVMAELAKELPQVSFVKLEAEGVPEVSEKYEISSVPTFLFFKNSQKIDRLDGAHAPELTKKVQRHASSGSFLSSANEHLKEDLNLRLKKLTHAAPCMLFMKGTPQEPRCGFSKQMVEILHKHNIQFSSFDIFSDEEVRQGLKAYSSWPTYPQLYVSGELIGGLDIIKELEASEELDTICPKAPKLEERLKVLTNKASVMLFMKGNKQEAKCGFSKQILEILNSTGVEYETFDILEDEEVRQGLKAYSNWPTYPQLYVKGELVGGLDIVKELKENGELLPILRGEN

相同性解析による交差性が予測される生物種
Full Name glutaredoxin 3
Calculated molecular weight 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC014372
Gene Symbol GLRX3
Gene ID (NCBI) 10539
RRIDAB_2110374
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO76003
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GLRX3(Glutaredoxin-3) is also named as PICOT, TXNL2. Mammalian glutaredoxin 3 is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals(PMID:20226171). Glrx3 could take a pivotal role in colon and lung cancer cells during the tumorigenesis, which is expressed in lung and colon cancers consistently and preferentially(PMID:19797004).

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