Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, human milk, mouse lung tissue, Jurkat cells, human placenta tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 13 publications below |
| IF | See 5 publications below |
Product Information
15804-1-AP targets GLRX in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8535 Product name: Recombinant human GLRX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-106 aa of BC005304 Sequence: MAQEFVNCKIQPGKVVVFIKPTCPYCRRAQEILSQLPIKQGLLEFVDITATNHTNEIQDYLQQLTGARTVPRVFIGKDCIGGCSDLVSLQQSGELLTRLKQIGALQ 相同性解析による交差性が予測される生物種 |
| Full Name | glutaredoxin (thioltransferase) |
| Calculated molecular weight | 106 aa, 12 kDa |
| Observed molecular weight | 12 kDa |
| GenBank accession number | BC005304 |
| Gene Symbol | GLRX |
| Gene ID (NCBI) | 2745 |
| RRID | AB_11232210 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35754 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GLRX antibody 15804-1-AP | Download protocol |
| WB protocol for GLRX antibody 15804-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Dihydrotanshinone I preconditions myocardium against ischemic injury via PKM2 glutathionylation sensitive to ROS | ||
Biomaterials Modeling calcific aortic valve disease with engineered human valve tissues identifies SAMHD1 as a therapeutic target. | ||
J Pharm Anal Naringenin boosts Parkin-mediated mitophagy via estrogen receptor alpha to maintain mitochondrial quality control and heal diabetic foot ulcer. | ||
J Pharm Anal Glutaredoxin-1 alleviates acetaminophen-induced liver injury by decreasing its toxic metabolites | ||
Antioxidants (Basel) Glutathione Induces Keap1 S-Glutathionylation and Mitigates Oscillating Glucose-Induced β-Cell Dysfunction by Activating Nrf2 | ||
Free Radic Biol Med Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.
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