Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse brain using 15371-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spinal cord using 15371-1-AP
mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 15371-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 15371-1-AP (GLRB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of mouse cerebellum using 15371-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 15371-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 15371-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 15371-1-AP
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 15371-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse cerebellum using 15371-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15371-1-AP (GLRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GLRB Polyclonal antibody

GLRB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15371-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

GLRB, Glycine receptor subunit beta, glycine receptor, beta

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue, mouse spinal cord tissue
Positive IHC detected in	mouse cerebellum tissue, rat cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

15371-1-AP targets GLRB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4429 Product name: Recombinant human GLRB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-267 aa of BC032635 Sequence: VEEAYSKEKSSKKGKGKKKQYLCPSQQSAEDLARVPANSTSNILNRLLVSYDPRIRPNFKGIPVDVVVNIFINSFGSIQETTMDYRVNIFLRQKWNDPRLKLPSDFRGSDALTVDPTMYKCLWKPDLFFANEKSANFHDVTQENILLFIFRDGDVLVSMRLSITLSCPLDLTLFPMDTQRCKMQLESFGYTTDDLRFIWQSGDPVQLEKIALPQFDIKKEDIEYGNCTKYYKGTGYYTCVEVIFTLRRQVG 相同性解析による交差性が予測される生物種
Full Name	glycine receptor, beta
Calculated molecular weight	56 kDa
Observed molecular weight	56 kDa
GenBank accession number	BC032635
Gene Symbol	GLRB
Gene ID (NCBI)	2743
RRID	AB_2878132
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P48167
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glycine is an inhibitory neurotransmitter in the central nervous system. The glycine receptor (GlyR) is a member of the nicotinic acetylcholine receptor of ligand-gated ion channels. This receptor has important roles in a variety of physiological processes, especially in mediating inhibitory neurotransmission in the spinal cord and brain stem. GlyR is a pentameric receptor comprised of alpha and beta subunits. Four alpha-subunits (GLRA1, GLRA2, GLRA3, GLRA4) and one beta-subunit (GLRB) of GlyR have been identified. (PMID: 11409700; 15383648)

Protocols

Product Specific Protocols
IHC protocol for GLRB antibody 15371-1-AP	Download protocol
WB protocol for GLRB antibody 15371-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	PLoS Biol mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice. Authors - Zi-Yang Zhang View Article
mouse	WB	Psychopharmacology (Berl) Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression. Authors - Niannian Huang View Article
mouse	WB	J Neurosci Spinal cord synaptic plasticity by GlyRβ release from receptor fields and syndapin I-dependent uptake Authors - Jessica Tröger View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 15371-1-AP

WB analysis of mouse spinal cord using 15371-1-AP

WB analysis of rat brain using 15371-1-AP

IHC staining of mouse cerebellum using 15371-1-AP

IHC staining of mouse cerebellum using 15371-1-AP

IHC staining of rat cerebellum using 15371-1-AP

IHC staining of rat cerebellum using 15371-1-AP

IHC staining of mouse cerebellum using 15371-1-AP

IHC staining of mouse cerebellum using 15371-1-AP

GLRB Polyclonal antibody

GLRB Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

mGluR5/ERK signaling regulated the phosphorylation and function of glycine receptor α1ins subunit in spinal dorsal horn of mice.

Contribution of skeletal muscular glycine to rapid antidepressant effects of ketamine in an inflammation-induced mouse model of depression.

Spinal cord synaptic plasticity by GlyRβ release from receptor fields and syndapin I-dependent uptake