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GLO1 Monoclonal antibody

GLO1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68242-1-Ig
Clone No.1B7A8

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

GLYI, Glx I, GLOD1, ELNR1, EC:4.4.1.5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, LNCaP cells, Jurkat cells, HepG2 cells, K-562 cells, HSC-T6 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68242-1-Ig targets GLO1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GLO1 fusion protein Ag7314 相同性解析による交差性が予測される生物種
Full Name glyoxalase I
Calculated molecular weight 21 kDa
Observed molecular weight 21-25 kDa
GenBank accession numberBC001741
Gene Symbol GLO1
Gene ID (NCBI) 2739
RRIDAB_2935329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04760
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glo1 (glyxoalase I) is a cytosolic protein expressed in all mammalian cells. Its physiological function is the detoxification of MG (methylglyoxal), which is a potent precursor of AGEs (advanced glycation end-products). Glo1 is known to play a role in many diseases including diabetes mellitus, Alzheimer's disease, and cancer.

Protocols

Product Specific Protocols
WB protocol for GLO1 antibody 68242-1-IgDownload protocol
IF protocol for GLO1 antibody 68242-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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